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a single possible rationalization to the amplified Aβ output during the Aph1 L30F/T164A mutant is that the mutations improve sophisticated formation. To test whether or not the Aph1 L30F/T164A mutants type a complex with other components, γ-secretase in yeast microsomes was immunoprecipitated using a PS1 antibody versus the loop location (GIL3) (determine five).
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This review discovered that Aph1aL may be actively linked to γ-secretase catalytic activity. We analyzed the creation of distinct Aβ species and found that the ratio of prolonged Aβ species (Aβ42 in addition to aβ43) which has aβ40 was similar in between the Aph1aL WT and L30F/T164A double mutant.
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